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KMID : 0363220100480020079
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Korean Journal of Dermatology
2010 Volume.48 No. 2 p.79 ~ p.86
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Collagen Synthesis and Expression of TGF-beta1 in Er:glass Fractional Laser Treated Hairless Mice
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Kim Sung-Ae
Lee Dae-Sic
Kim Guang-Hoon
Kim Sergey
Lee Kyu-Suk
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Abstract
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Background: Non-ablative dermal remodeling and ablative skin resurfacing are currently well-established skin treatment modalities. Fractional laser was recently introduced as a new concept for laser skin rejuvenation, and is characterized by creation of a dense pattern of epidermal and dermal microthermal treatment zones (MTZs). However, the precise mechanisms of dermal remodeling by Er:glass fractional laser treatment are largely unknown.
Objective: The purpose of this study was to investigate the effect of 1,550 nm Er:glass fractional laser treatment on dermal collagen synthesis and expression of TGF-β1, a potent cytokine involved in collagen synthesis.
Methods: We treated hairless mice with several power densities (5 W 5 mJ∼20 W 20 mJ), and examined the tissue samples on days 1, 30, and 90 after treatment. We analyzed the penetrating depth of laser treatment by determining dermal response through assessment of type I collagen synthesis and TGF-β1 expression by H&E, Masson-trichrome staining, western blot analysis and immunohistochemistry staining.
Results: We observed, through H&E staining, that increasing the pulse energy of fractional laser treatment correlated with increasing depth of MTZ. Also, fractional laser treatment increased type 1 collagen synthesis on days 30 and 90, energy dependently. Immunohistochemical study showed that fractional laser treatment increased expression of type I collagen and TGF-β1, energy dependently, with TGF-β1 expression peaking on day 1. In addition, according to western blot analysis, expressions of TGF-β1 and type I collagen were up-regulated in an energydependent manner.
Conclusion: Er:glass fractional laser induced dermal remodeling by up-regulation of TGF-β1 and type I collagen synthesis, and may be a promising modality for skin rejuvenation. (Korean J Dermatol 2010;48(2):79∼86)
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KEYWORD
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Fractional laser, TGF-β1, Type I collagen
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